Technical Data

G3500-19C
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
10E23(D25G9)
Grade
Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Gene ID
2892
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GluR3 (Glutamate Receptor 3, GluR3, GluR-3, AMPA-selective Glutamate Receptor 3, AMPA3, GLURC, GluR-C, GluR-K3, Ionotropic Glutamate Receptor AMPA 3, Gria3, MRX94)

AMPA- (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers and mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization and plasticity. Post-transcriptional modifications (alternative splicing and nuclear RNA editing) and post-translational modifications (glycosylation, phoshorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs (1). GluR 3 knockout mice exhibited normal basal synaptic transmission and long-term depression (LTD) but enhanced long-term potentiation (LTP). In contrast, GluR 2/3 double knockout mice are impaired in basal synaptic transmission (2). GluR 3 is implicated in a number of diseases including autoimmune epilepsy, X-linked mental retardation, Rett’s syndrome, amyotrophic lateral sclerosis (ALS) and Alzheimer’s disease (3).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding His70 of human GluR 3.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recongnizes endogenous levels of human total human GluR 3. Species crossreactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77, 2. Meng, Y. et al. (2003) Neuron 39, 163-76, 3. Rembach, A. et al. (2004) J Neurosci Res 77, 573-82.
USBio References
No references available
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