Mouse Anti-GLUT 2 (Glucose Transporter, Insulin Regulatable)
GLUT 2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. GLUT 2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 0.25ug labels 1x10e6 HepG2 human hepatocellular carcinoma cell line Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (medulla), liver, normal pancreas array and pancreatic cancer tissue Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Met1-Val524 from human GLUT 2 expressed in NS0 cells.
Form
Supplied as a liquid in PBS, pH 7.4, 5% trehalose.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on human GLUT 2-transfected NS0 cells, but not on control transfectants. Detects GLUT 2 on the surface of the human intestinal cell line Caco-2 in Flow Cytometry and on fixed cells in Immunocytochemistry. Based on Flow Cytometric tests on transfected cells, does not crossreact with human GLUT1 or GLUT3.
