Rabbit Anti-GLUT4 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 4, Glucose Transporter Type 4, Insulin-responsive, GLUT-4, SLC2A4)
Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter . It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes.Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.
Applications
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry (paraffin): 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to the C-terminal of human glucose transporter 4, identical to the related rat and mouse sequence.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GLUT4. Species Cross-Reactivity: mouse, rat.
