Technical Data

G3900-65B-FITC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
FITC
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
Rabbit Anti-GLUT 5 (Glucose Transporter 5, Glucose Transporter Type 5 Small Intestine, GLUT5, GLUT-5, Fructose Transporter, Solute Carrier Family 2 Facilitated Glucose Transporter Member 5, SLC2A5) (FITC)

Glucose 5 transporter catalyses the uptake of D-fructose into cells. This 55kD protein is expressed in the plasma membrane and is found mainly in intestinal tissue, kidney, and spermatozoa. Increased intestinal levels of GLUT5 are seen in type 2 diabetes

Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1:50-1:200 Western Blot: 1:150-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
A synthetic peptide (ELKELPPVTSEQ) corresponding to the carboxy terminus of the Human GLUT 5. To accomodate KLH conjugation a cysteine was added to the N-terminal.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Glucose Transporter 5. Does not crossreact with mouse or rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Mahraoui, L. et al. (1992) Expression and localization of GLUT-5 in Caco-2 cells, human small intestine, and colon. Am. J. Physiol. 263: G312-G318.
USBio References
No references available
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