Rabbit Anti-GLUT 5 (Glucose Transporter)
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-60kD) and Sodium glucose co-trasporter-1 (SGLT-1, 662aa, ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1-10ug/ml using ECL. The antibody has been shown to react with a protein band of about 60kD in the rat jejunum membranes that is slightly larger than that reported in human GLUT 5 (50-55kD). In rat kidney, a protein of ~42kD was detected. ELISA: 1:1000- 1:10,000. Use G3900-65G control peptide to coat ELISA plates at 1ug/ml. Immunohistochemistry: See reference (2). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresonding to 13aa near the C-terminal, cytoplasmic domain of rat Glut-5, conjugatd to KLH. Species Sequence Homology: human: 61%, mouse: 53%.
Form
Supplied as liquid in PBS, pH 7.4, 0.1% BSA.
Purity
Purified by affinity chromatography.
Specificity
Recognizes rat Glut-5.
