Mouse Anti-Glutamate Decarboxylase 67 (GAD67) (APC)
Glutamic acid decarboxylase (GAD) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% amino acid identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. GAD67 is cytoplasmic (594aa), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation or disease progression.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Paraffin sections. No epitope retrieval necessary. Optimal dilutions to be determined by the researcher.
Positive Control
SKNSH cell lysate (human neuroblastoma), mouse cerebral cortex
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to rat GAD67
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes rat Glutamate Decarboxylase 67 at ~67kD. No detectable crossreactivity with GAD65 by Western Blot on rat brain lysate. Species Crossreactivity: human and mouse
