Technical Data

G4003-90C
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Serum
Applications
IC IHC WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Glutamate-aspartate Transporter (GLAST1, EAAT1 GLAST, EA6, Excitatory Amino Acid Transporter 1, FLJ25094, Glial High Affinity Glutamate Transporter, High Affinity Neuronal Glutamate Transporter, Slc1a3, Sodium Dependent Glutamate/aspartate Transporter) (Not for Export EU)
GLAST1, EAAT1 GLAST, EA6, Excitatory amino acid transporter 1, FLJ25094, Glial high affinity glutamate transporter, High affinity neuronal glutamate transporter, Slc1a3, Sodium dependent glutamate/aspartate transporter
Applications
Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
20 residue C-terminal synthetic peptide (Rat).
Form
Lyophilized. Whole Serum
Purity
Serum
Specificity
This antibody reacts with Glutamate-aspartate transporter in CNS samples. Species Crossreactivity: Crossreacts with Rat. The% of homology for the sequence used during the development of antibodies is 85% (between GLAST from rat and GLAST from human).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kobayashi S & Millhorn DE Hypoxia regulates glutamate metabolism and membrane transport in rat PC12 cells. J Neurochem 76:1935-48 (2001). <PUBMED:11259512> Schmitt A et al. Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry. J Neurosci 17:1-10 (1997). <PUBMED:8987731>Rothstein JD et al. Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate. Neuron 16:675-86 (1996). <PUBMED:8785064>
USBio References
No references available
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