Technical Data

G4003-90W
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Glutamate Transporter, Aspartate EAAT1 (GLAST1, GLAST-1, GLAST, EA6, Excitatory Amino Acid Transporter 1, EAAT1, FLJ25094, Glial High Affinity Glutamate Transporter, High Affinity Neuronal Glutamate Transporter, Sodium Dependent Glutamate/aspartate Transporter 1, Solute Carrier Family 1 Member 3, SLC1A3)
Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3

Excitatory amino acid transporter 1 (EAAT1) is a glutamate transporter (GluT). It rapidly removes L-glutamate from the synaptic cleft which is essential for terminating the postsynaptic action of glutamate. EAAT1 is also known as high affinity sodium-dependent glutamate/aspartate transporter 1 (GLAST1). EAAT1 is highly expressed in cerebellum but is also found in the frontal cortex, hippocampus and basal ganglia.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1.0 ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa516-531, ENEMKKPYQLIAQDNE to the C-terminus of human EAAT1/GLAST1.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human EAAT1/GLAST1 at 60kD. Species Crossreactivity: mouse, rat, rabbit.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Sinha, D., et,al. (2005) Am J Physiol Renal Physiol ; 288: F703 - F713, 2.Onofri, F., et,al. (2000) J. Biol. Chem ; 275: 29857. 3.Bonanomi, D., et,al. (2005) J. Neurosci; 25: 7299 - 7308, 4.Tokumitsu, H., et,al. (2005) J. Biol. Chem ; 280: 35108 - 35118.
USBio References
No references available
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