Technical Data

G4005-91
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Glutamate Transporter EAAT2 (GLT1)

Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573 AA) display about 55% homology and are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and purkinje cells, and specifically enriched in cortex, hippocampus, and caudate-putamen and confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
Western Blot: 1-10ug/ml using Chemiluminescence technique. ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 16aa from the cytoplasmic, N-terminus of rat GLT1 (P31596), conjugated to KLH. Species Sequence Homology: mouse (100%); human (81%).
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Glutamate Transporter. Has no significant sequence homology to other glutamate transporters.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Pines, G. et al (1992) Nature 360:464-467. 2. Kanner, BI et al (1993) FEBS Lett. 325, 95-99). 3. Zhang, Y. et al (1994) J. Biol. Chem. 269:19573-19577. 4. Grunewald, M. and Kanner, B. (1995) J. Biol. Chem. 270:17017-17024. 5. Arriza, J. L. et al (1994) J. Neurosci 14:5559-5569. 6. Krischner, M. A. et al (1994) Genomics 24:218-224. 7. Zhang, Y. et al (1994) J. Biol. Chem. 269:19573-19577. 8. Grunewald, M. and Kanner, B. (1995) J. Biol. Chem. 270:17017-17024
USBio References
No references available
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