Technical Data

G8135-04G-APC
Clone Type
Polyclonal
Host
Rabbit
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
BSA Free
Rabbit Anti-Glutathione S-Transferase (GST) (APC)

pGEX vectors are frequently utilized in bacterial systems to express polypeptides or entire proteins as fusions with glutathione S-transferase. Using this fusion system, a polypeptide sequence is linked to the C-terminus of the 26 kD GST protein from Schistosoma japonicum. The bacterially expressed GST-fusion proteins are purified by absorption with glutathione-agarose beads. The fusion protein is subsequently eluted from the glutathione agarose with an excess of free glutathione. Antibodies to GST are used to detect pGEX-expressed fusion proteins and as immuno-affinity reagents for purification of recombinant proteins.

Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
FLISA (solid phase = antigen): 0.1-1.0ug/ml FLISA (solid phase = antibody): 1.0ug/ml Western Blotting: 1ug/ml Optimal dilution determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified glutathione S-transferase (GST) protein from Schistosoma japonicum expressed from a pGEX vector.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by affinity chromatography.
Specificity
Specific for the ~26kD GST protein from S. japonicum and is useful for detecting GST and GST-fusion proteins transcribed from pGEX and similar vectors. Cross-reactivity with endogenous proteins from E. coli bacteria has not been observed.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Smith, D.B., and Corcoran, L.M. (1991). Expression and Purification of Glutathione-S-Transferase Fusion Proteins In: Current Protocols in Molecular Biology (Unit 16.7). Green Publishing Assoc. Inc, and John Wiley & Sons Inc. 2. Smith, D.B., Davern, K.M., Board, P.G., et. al.; Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proc. Natl. Acad. Sci. USA 83:8703-8707 (1986). 3. Smith, D.B. and Johnson K.S. (1988). Single-step purification of polypeptides in Escherichia coli as fusions with glutathione Stransferase. Gene 67:31-40.
USBio References
No references available
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