Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).

Suitable for use in FLISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested.

Western Blot: Detects on 10ug of A431 lysates. Immunocytochemistry: Dilute in PBS, 10mg/ml BSA. Fixative: 4% PFA, 0.02% PBS-Triton X-100. Incubation time: 3 minutes at RT. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.    

Note: Applications are based on unconjugated antibody.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography

Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle at ~38kD. Also recognizes cardiac GAPDH. Species Crossreactivity: human, porcine, canine, feline, rat, mouse and fish. Does not react with GAPDH from E. coli. Also detected in many non-muscle cell lines including HeLa, HCT-116, U937, and THP-1 cells.