Technical Data

G8140-15D
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_002037
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GAPDH, NT (Glyceraldehyde-3-phosphate dehydrogenase, EC 1.2.1.12, GAPD, CDABP0047, OK/SW-cl.12)

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-terminal region of human GAPDH (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human GAPDH.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Azam,S., J. Biol. Chem. 283(45), 30632-30641 (2008). 2.Lu,J., Biosci. Biotechnol. Biochem. 72(9), 2432-2435 (2008). 3.Zhou,Y., Mol. Cancer Res. 6(8), 1375-1384 (2008).
USBio References
No references available
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