Rabbit Anti-Glycine Receptor alpha 1/2 (HRP)
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation.
Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: frozen Optimal dilution determined by the researcher
Recommended Secondary Reagents
Sheep anti rabbit IgG:HRP
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a region within the amino terminus of the alpha 1 subunit of rat glycine receptor.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes the alpha 1 and alpha 2 subunits of rat glycine receptor. Species Crossreactivity: mouse and human
