Technical Data

G8168-23
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Glycine Transporter-2 (GLYT2) (Glycerol Free)

The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms-GLYT1 and GLYT2. Form 1 has three other known isoforms (GLYT1a, GLYT1b, and GLYT1c) which may be produced by alternative splicing or promoter usage. Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and are expressed from the same gene. Although the N-terminal part of mouse and rat GLYT1a is identical in mouse and rat, the N-terminus of GLYT1b is significantly different in these two species. The GLYT1c subtype has only been reported in humans. GLYT1a is expressed in CNS and peripheral organs. GLYT1b is localized in the CNS. Both neuron and Glial cells have GLYT1. More recent studies indicate that GLYt1b may not be brain specific. Immunolocalization studies on GLYT2 suggest that GLYT2 is responsible for terminating the neurotransmission at the strychnine synapses.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml using ECL. ELISA: 1:10,000-1:50,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher.
Control Peptide: G8168-25
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide consisting of 20aa sequence within the cytoplasmic, C-terminal domain of rat GLYT2. Species Sequence Homology: human and mouse.
Form
Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide. Glycerol free.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat GLYT2. No homology is seen with either GLYT1 or other transporters.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Liu, Q.R., et al., JBC 268: 22802 (1993). 2. Jursky, F., et al., J. Neurochem. 67: 336(1996). 3. Liu, Q.R., Nelson, H., Mandiyan, S., Lopez-Corcuera, B., Nelson, N.: Cloning and expression of a glycine transporter from mouse brain. FEBS Letts. 305: 110-114 (1992). 4. Borowsky, B., Mezey, E., Hoffman, B.J.: Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene. Neuron 10: 851-863 (1993). 5. Kim, K.M., Kingsmore, S.F., Han, H., Yang-Feng, T.L., Godinot, N., Seldin, M.F., Caron, M.G., Giros, B.: Cloning of the human glycine transporter type 1: molecular and pharmacological characterization of novel isoform variants and chromosomal localization of the gene in the human and mouse genomes. Mol. Pharmacol. 45: 608-617 (1994). 6. Adams, R.H., Sato, K., Shimada, S., Tohyama, M., Puschel, A.W., Betz, H.: Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents. J. Neurosci. 15: 2524-2532 (1995). 7. Evans, J., Herdon, H., Cairns, W., O'Brien, E., Chapman, C., Terrett, J., Gloger, I.: Cloning, functional characterisation and population analysis of a variant form of the human glycine type 2 transporter. FEBS Letts. 463: 301-306 (1999). 8. Horiuchi, M., Nicke, A., Gomeza, J., Ashcrafi, A., Schmalzing, G., Betz, H.: Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopusoocytes. PNAS USA 98: 1448-1453 (2001).
USBio References
No references available
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