G8170-44F
Clone Type
PolyclonalHost
RabbitSource
RatIsotype
IgGGrade
Affinity PurifiedApplications
IC IHC WBCrossreactivity
Bo Hu Mo RtShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Glycogen Synthase Kinase 3 beta (GSK3b)
GSK3 beta, also known as Tpk1 (tau protein kinase-1), is a 47kD protein-serine/threonine kinase with broad specificity that phosphorylates and inactivates glycogen synthase, the phosphatase 1 inhibitor protein, and other proteins that feature a (S/T)PXX(Tp/Sp) recognition motif. It is ubiquitously distributed, but highly expressed in mammalian brain, and occurs in two isoforms, 51kD (alpha) and 47kD (beta). This monomeric proline-directed kinase is inhibited when it is phosphorylated by protein kinase B and S6 protein kinases. It is activated when it is phosphorylated by an unknown protein-tyrosine kinase. GSK3beta is inhibited in response to insulin. GSK3beta is phosphorylated and inactivated by protein kinase C, p70, S6 kinase and Rsk-1.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot (Colorimetric): 1:1000 Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by researcher.
Positive Controls
HeLa Cell Lysate, Mouse Brain Tissue Extract Rat Brain Tissue Extract
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa335-349, CAHSFFDELRDPNVK from rat GSK3b, conjugated to KLH.
Form
Supplied as a liquid PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein A affinity chromatography from serum
Specificity
Recognizes rat GSK3b at ~47kD. Species Crossreactivity: human, mouse and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Woodgett, J.R. (1990) EMBO J. 9: 2431-2438. 2. Behrens, J., Jerchow, B.-A., Würtele, M., Grimm, J., Asbrand, C., Wirtz, R., Kühl, M., Wedlich, D. and Birchmeier, W. (1998) Science 280: 596-599. 3. Ishiguro, K. et al (1993) FEBS Lett. 325: 167-172. 4. Hardie, G. and Hanks, S. (1995) The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418ppsUSBio References
No references available