Technical Data

G8235-52J
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Glypican 4 (K-glypican, UNQ474/PRO937, GPC4)

Glypican-4, previously known as K-glypican, is a GPI-linked cell surface heparan sulfate proteoglycan of the glypican gene family, that comprises 6 members at present. Its function is only partially understood at present. However, glypican-4 seems to be involved in the control of cell growth and diffe-rentiation, especially in the development of the central nervous system and the kidney. Furthermore, glypican-4 is expressed by haematopoetic stem cells and bone marrow stromal cells. Although the antiserum was prepared against a peptide from hu man glypican-4, due to the highly conserved structure and se-quence of glypican-4, antiserum anti-peptide 1 (aa88-101) was used successfully to study brain development in the mouse.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 5-10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to internal domain of human GPC4 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GPC4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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