Goat Anti-G-Protein beta Subunit-like (GbL)
G-protein β-subunit-like (GβL), also known as mLST8, is a subunit of the Target of Rapamycin (TOR) complex. GβL contains 7 WD-40 repeats, and binds to the kinase domain of TOR in both the Raptor (Rapamycin-sensitive)-and Rictor (Rapamycin- insensitive)-containing complexes. GβL is necessary for the full activation of the TOR kinase, and phosphorylation of many downstream proteins, including 4E-BP1 and p70 S6 kinase.
Applications
Western Blot: An antibody concentration of 1.0ug/ml is recommended.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived recombinant human GbL (aa1-327)
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Detects human, mouse and rat GbL.
