Mouse Anti-G-Protein Coupled Receptor 77 (GPCR77, GPR77, C5L2, C5a Anaphylatoxin Chemotactic Receptor)
C5L2 (C5a receptor-like 2) is a seven-transmembrane orphan receptor, also known as GPR77, which shares significant homology to C5a receptor (CD88). But it is a decoy receptor without G-protein coupling. C5L2 is co-expressed with C5aR on many kinds of cells, such as neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes. C5L2 binds C5a with similar high affinity as C5aR and relatively higher affinity for C5a-des-Arg than with C5aR. This antibody is known to block C5a binding to C5L2, but does not react with C5a receptor.
Applications
Suitable for use in Flow Cytometry. Not recommended for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤2ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human C5L2.
