Technical Data

G8750-02A
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
FITC
Isotype
IgG2b,k
Clone Number
RB6-8C5
Grade
Purified
Applications
FC IHC IP WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Gr-1 (GR1, Myeloid Differentiation Antigen, Ly-6G) (FITC)

Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein (1). In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow (2). In the periphery, Ly-6G is expressed predominantly on neutrophils (3, 4).

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot and In vitro activation of complement-dependent cytoxicity Assays. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤1ug/10e6 cells. Immunohistochemistry: frozen sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Immunogen
Normal mouse bone marrow cells.
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Purity
Purified.
Specificity
Recognizes mouse Gr-1 at ~21-25kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fleming, T.J., M.L. Fleming and T. R. Malek. 1993. J. Immunol. 151:2399. 2. Hestdal, K., F.W. Ruscetti, J.N. Ihle, et al. 1991. J. Immunol. 147:22. 3. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313. 4. Lagasse, E., and I.L. Weissman. 1996. J. Immunol. Meth. 197:139. 5. Southern Biotechnology Associates, Inc. Unpublished observations. 6. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, et al. 1988. Eur. J. Immunol. 18:1819. 7. Brummer, E., A.M. Sugar, and D.A. Stevens. 1984. J. Leukocyte Biol. 36:505. 8. Stoppacciaro, A., C. Melani, M. Parenza, et al. 1993. J. Exp. Med. 178:151. 9. Tumpey, T.M., S.-H. Chen, J.E. Oakes, and R.N. Lausch. 1996. J. Virol. 70:898. 10. Conlan, J.W., and.J. North. 1994. J. Exp. Med. 179:259. 11. Czuprynski, C.J., J.F. Brown, N. Maroushek, R.D. Wagner, and H. Steinberg. 1994. J. Immunol. 152:1836. 12. Rakhmilevich, A.L. 1995. J. Leukoc. Biol. 57:827.
USBio References
No references available
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