Technical Data

G8750-02D
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
APC
Isotype
IgG2b,k
Clone Number
RB6-8C5
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Rat Anti-Gr-1 (GR1, Myeloid Differentiation Antigen, Ly-6G) (APC)

Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. In the periphery, Ly-6G is expressed predominantly on neutrophils.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: ≤0.1ug/10e6 cells Immunohistochemistry: Frozen sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with Allophycocyanin (APC).
Purity
Purified
Specificity
Recognizes mouse Ly-6G and Ly-6C.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fleming, T.J., M.L. Fleming and T. R. Malek. 1993. J. Immunol. 151:2399.|2. Hestdal, K., F.W. Ruscetti, J.N. Ihle, et al. 1991. J. Immunol. 147:22.|3. Lewinsohn, D.M., R.F. Bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313.|4. Lagasse, E., and I.L. Weissman. 1996. J. Immunol. Meth. 197:139.|5. Southern Biotechnology Associates, Inc. Unpublished observations.|6. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, et al. 1988. Eur. J. Immunol. 18:1819.|7. Brummer, E., A.M. Sugar, and D.A. Stevens. 1984. J. Leukocyte Biol. 36:505.|8. Stoppacciaro, A., C. Melani, M. Parenza, et al. 1993. J. Exp. Med. 178:151.|9. Tumpey, T.M., S.-H. Chen, J.E. Oakes, and R.N. Lausch. 1996. J. Virol. 70:898.|10. Conlan, J.W., and.J. North. 1994. J. Exp. Med. 179:259.|11. Czuprynski, C.J., J.F. Brown, N. Maroushek, R.D. Wagner, and H. Steinberg. 1994. J. Immunol. 152:1836.|12. Rakhmilevich, A.L. 1995. J. Leukoc. Biol. 57:827.
USBio References
No references available
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