Technical Data

G8951-10C
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a,k
Clone Number
MP1-22E9
Grade
Purified
Applications
E WB
Crossreactivity
Mo
Accession #
NP_034099
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rat Anti-Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim)
Applications
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse GM-CSF protein levels. Purified antibody should be paired with biotinylated antibody (Cat. No. G8951-13C) as the detection antibody, with purified recombinant mouse GM-CSF as the standard. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of ≤ 100ng/lane of recombinant mouse GM-CSF under reducing conditions.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Yeast-expressed recombinant mouse GM-CSF
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity
Purified
Specificity
Recognizes mouse Granulocyte Macrophage Colony Stimulating Factor (GM-CSF).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.|2. Suda, T., A. O’Garra, I. MacNeil, M. Fischer, M. Bond, and A. Zlotnik. 1990. Cell. Immunol. 129:228.|3. Nozaki, S., J. Abrams, M. Pearce, and D. Sauder. 1991. J. Invest. Dermatol. 97:10.
USBio References
No references available
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