Mouse Anti-GMR-alpha (CD116, Granulocyte-macrophage Colony-stimulating Factor Receptor Subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, CDw116, CSF2RA, CSF2R, CSF2RY)
CD116 is a 70-85kD a chain of the GM-CSF receptor. It combines with CDw131 B chain to form the high affinity GM-CSF receptor. A soluble form of CD116, which binds GM-CSF with a relatively low affinity, has been identified. In addition, an alternatively spliced form of CD116 with an altered cytoplasmic tail has been described. CD116 is expressed on various myeloid cells including monocytes, macrophages, neutrophils, eosinophils, dendritic cells and their precursors, fibroblasts, and endothelial cells. CD116 is expressed on myeloid leukemias, osteogenic sarcoma cell lines, osteoblast-like cells and breast and lung carcinoma cell lines.
Applications
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤0.5ug/10e5 cells in 100ul Immunohistochemistry: acetone-fixed, frozen tissue sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human GM-CSFRa transfected COS cells
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD116.
