Goat Anti-Green Fluorescent Protein (GFP)
Green fluorescent protein (GFP) is a 27kD protein which is derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression.
Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000-1:30,000 Western Blot: 1:400-1:10,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein corresponding to full length GFP derived from Aequorea Victoria.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes wild type GFP, but will also detect recombinant and enhanced forms of GFP.
