G8972-15-ML550
Clone Type
MonoclonalHost
MouseSource
BovineConjugate
MaxLight™550Isotype
IgG1,kClone Number
A16/17Grade
PurifiedApplications
FLISA IP WBCrossreactivity
Bo Hu Mo RtShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-GRK 4-6 (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Applications
Suitable for use in Western Blot, Immunoprecipitation, FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
GST fusion protein corresponding to residues 463-590 of bovine GRK 5.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes GRK 4, GRK 5 and GRK 6. Species Crossreactivity: Human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
General Freedman, N.J., et al., J. Biol. Chem. 272: 17734-11743, 1997. Pitcher, J.A., et al., J. Biol. Chem. 271: 24907-24913, 1996. Nagayama, Y., et al., J. Biol. Chem. 271: 10143-10148, 1996. Application Oppermann, M., et al., Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996.USBio References
No references available