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G8975-22C Rabbit Anti-GRO beta (Growth-regulated Protein beta, Gro-beta, GROb, Chemokine (C-X-C Motif) Ligand 2, CXCL2, C-X-C Motif Chemokine 2, CINC-2a, GRO2, Macrophage Inflammatory Protein 2-alpha, MIP2-alpha, MIP2A, MIP-2a, MIP2, MGSA-b, SCYB2)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Hematopoietic Synergistic Factor, HSF, GRO-beta-T, SB-251353

GRO-beta, otherwise known as CXCL2, is a 7.9kD CXC chemokine and one of three closely related, but distinct GRO gene products, which act as chemoattractants and activators of neutrophils and basophils.

Like GRO-alpha (CXCL1) and GRO-gamma (CXCL3), GRO-beta contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors, though studies have shown that GRO-beta has a lower potency and receptor binding affinity.
Applications
Suitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human GRO-beta.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GRO-beta.
References
1. Haskill, S. et al. (1990) Identification of three related human GRO genes encoding cytokine functions. P.N.A.S. 87: 7732-7736. 2. Geiser, T. et al. (1993) The Interleukin-8-related chemotactic cytokines GROalpha, GRObeta, and GROgamma activate human neutrophil and basophil leukocytes. J. Biol. Chem. 268: 15419-15424.
USBio References
No references available
Conjugates
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