Mouse Anti-Human Growth Hormone (hGH) (APC)
Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191aa residues. The 20kD variant form of human growth hormone (20kD hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kD, 191aa) by the deletion of aa residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kD hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Matched Pairs
Capture: G9000-03B Detection: G9000-03D
Affinity Constant
1x10e10 L/mole
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Allophycocyanin (APC) is light sensitive.
Note: Applications are based on unconjugated antibody.
Immunogen
hGH isolated from human pituitary gland.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes hGH. Cross-reactivity: Human Placental Lactogen: <0.02%; Human Prolactin: <0.02%.
