Mouse Anti-Human Growth Hormone (hGH)
Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191aa residues. The 20kD variant form of human growth hormone (20kD hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kD, 191aa) by the deletion of aa residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kD hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Affinity Constant
1x10e10 L/mole
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
hGH isolated from human pituitary gland.
Form
Supplied as a liquid in 10mM phosphate, pH 7.4, 150mM sodium chloride, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes hGH. Cross-reactivity: Synthetic hGH (Somatonorm): 100%; Human Placental Lactogen: <0.02%; Human Prolactin: <0.02%.
