Technical Data

G9804-47
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_000846
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Guanylate Cyclase alpha-2, Soluble, CT (Guanylate Cyclase Soluble Subunit alpha-2, Guanylate Cyclase 1 Soluble alpha 2, GCS-alpha-2, GC-SA2, GUC1A2, GUCSA2, GUCY1A2)
EC=4.6.1.2

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:200 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human GUCY1A2 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human GUCY1A2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Baba, H., et al., Neuroreport 15(4):677-680 (2004). 2.Bellamy, T.C., et al., Proc. Natl. Acad. Sci. USA 99(1):507-510 (2002). 3.Russwurm, M., et al., J. Biol. Chem. 276(48):44647-44652 (2001). 4.Bamberger, A.M., et al., J. Clin. Endocrinol. Metab. 86(2):909-912 (2001). 5.Budworth, J., et al., Biochem. Biophys. Res. Commun. 263(3):696-701 (1999).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net