Technical Data

H1200-01
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Serum
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HA-Tag, fusion tag (HA Epitope Tag, HA Tag, Hemagglutinin) (Not for Export EU)

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000 (ECL). Recognizes native and denatured his-tag containing proteins. ELISA: 1:10,000-1:50,000. Coat with 50-100ng control antigen/well. Optimal dilutions to be determined by the researcher.
Control Peptide
H1200-02: HA epitope, fusion tag, Control Peptide
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Also Available
H1200-01: HA epitope, fusion tag Pab Rb xRb H1200-02: HA epitope, fusion tag, Control Peptide H1200-03: HA epitope, fusion tag Mab Mo x H1400: HA epitope, fusion tag Pab Rb x H1847-51: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Ch x H1847-51B: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (FITC) Mab Mo x H1847-52: Hemagglutinin Tag (CYPYDVPDYASL) (HA Tag) Pab Rb x H1847-52A: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Ch x H1847-52B: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Gt x H1847-52C: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Rb x H1847-52D: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (FITC) Pab Gt x H1847-52E: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (HRP) Pab Gt x H1847-52F: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (HRP) Pab Rb x H1847-52G: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (Agarose) Pab Gt x H1847-52X: Hemagglutinin Tag (CYPYDVPDYASL) (HA Tag) (BSA & Azide Free) Pab Rb x H1847-53: Hemagglutinin Tag (YPYDVPDYA) (HA tag) Pab Rb x H1847-53A: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Mab Mo x H1847-53X: Hemagglutinin Tag (YPYDVPDYA) (HA tag) Mab Mo x H1847-54: Hemagglutinin Tag (CYPYDVPDY) (HA tag) Mab Rb x H1847-55: Hemagglutinin Tag (YPYDVPDYA) (HA tag) Mab Mo x I7650-17A: Hemagglutinin (HA) EPITOPE TAG (IRDye™800) Pab Rb x I7650-17B: Hemagglutinin (HA) Epitope Tag (Biotin) Pab Rb x I7650-17C: Hemagglutinin (HA) EPITOPE TAG (IRDye™800CW) Pab Rb x
Immunogen
Synthetic peptide containing the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) protein (KLH coupled).
Form
Supplied as a liquid, neat serum, 0.05% sodium azide, 40% glycerol.
Purity
Serum
Specificity
Recognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA. Recognizes the HA-tag fused to either the N- or C-termini of targeted proteins. Species Crossreactivity: A broad species crossreactivity is expected based on conservation of sequence homology.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Terpe, K., Appl. Microbiol. Biotechnol. 60: 523-533 (2003). 2. Tu, Y., et al., Mol. Biol. Cell 9: 3367-3382 (1998). 3. Bhattacharya and Cabral, Mol. Biol. Cell 15(7): 3123-3131 (2004). 4. Yamanaka, et al., Curr. Biol. 13(9): 734-743 (2003). 5. Chang, et al., Genes Dev. 18(16): 2010-2023 (2004). 6. Munro, S., Pelham, H.R., EMBO J. 3: 3087-3093 (1984). 7. Madeo, F., et al., Mol. Cell 9: 911-917 (2002). 8. Ruckdeschel, K., et al., J. Immunol. 168: 4601-4611 (2002). 9. Field, J., et al., Mol. Cell. Biol. 8: 2159-2165 (1988). 10. Schirmer, E., et al., J. Cell Biol. 153: 479-490 (2001). 11. Bjerling, P., et al., Mol. Cell. Biol. 8: 2170-2181 (2002). 12. Wilson, l.A., et al., Cell 37: 767-78 (1984). 13. Muster, T., et al., J. Virol. 69: 6678-6686 (1995).
USBio References
No references available
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