Technical Data

H1741
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IF IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Hakai

Hakai is a c-Cbl-like protein. Hakai is an E-cadherin binding protein, which has been identified as an E3 ubiquitin-ligase. Hakai contains SH2, RING, zinc-finger and proline-rich domains. Hakai interacts with E-cadherin in a tyrosine phosphorylation-dependent manner, inducing ubiquitination of the E-cadherin complex. Expression of Hakai in epithelial cells disrupts cell-cell contacts and enhances endocytosis of E-cadherin and cell motility. By promoting the endocytosis and dynamic recycling or destruction of E-cadherin complexes, Hakai is able to control epithelial-mesenchymal transitions under physiological and pathological conditions.

Applications
Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:50 Immunofluorescence: 1:50 Western Blot: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human Hakai
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Hakai.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fujita, Y et al; Hakai, a c-Cbl-like protein, ubiquitinates and induces endocytosis of the E-cadherin complex. Nat Cell Biol 2002, 4: 222
USBio References
No references available
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