Technical Data

H1827-53A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Supernatant
Applications
E IC IF WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
3066
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HDAC2 (Histone Deacetylase 2, Tanscriptional Regulator Homolog RPD3, HD2)

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression (8-10). Both proteins are found together in numerous complexes including the nucleosome remodeling and deacetylation complex (NuRD), MeCP1, and the mSin3A corepressor complex.
Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:100 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to the C-terminus of the human HDAC2 protein.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Supernatant.
Specificity
Detects endogenous levels of HDAC2 protein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693-703. 2. Gregory, P.D. et al. (2001) Exp. Cell Res. 265, 195-202. 3. Liu, Y. et al. (2000) Mol. Cell. Biol. 20, 5540-5543. 4. Cress, S.D. and Seto, E. (2000) J. Cell. Physiol. 184, 1-16. 5. Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262, 75-83. 6. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84-100. 7. Viguishin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218. 8. Zhang, Y. et al. (1999) Genes Dev. 13, 1924-1935. 9. Ng, H.H. et al. (1999) Nat. Genet. 23, 58-61. 10. Zhang, Y. et al. (1997) Cell 89, 357-364.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net