Technical Data

H1832-98
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Purified
Applications
IC IHC IP WB
Crossreactivity
Hu Mo Rt
Accession #
NM_007355.2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Heat Shock Protein 90b (HSP84)
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 5ug/ml Immunohistochemistry (frozen and formalin/paraffin): 5-10ug/ml. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes. Immunoprecipitation: 10ug/mg of protein lysate (using Protein A) Optimal dilutions to be determined by the researcher.
Positive Control
MAD109 cells or breast carcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa2-13 from the N-terminus of mouse heat shock protein 90 beta. Cellular localization: Nuclear and Cytoplasmic.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes heat shock protein 90 beta (HSP90b). No reactivity with HSP90a/HSP86. Species Crossreactivity: rat, mouse and human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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