Rabbit Anti-Heat Shock Protein 40 (HSP40, HDJ1)
HSP40, also known as HDJ1 (9), is a basic mammalian 40kD heat shock protein which is homologous to the bacterial heat shock protein, DnaJ, and yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (1,2,3,4). Like many heat shock proteins, HSP40 is inducible by heat, transient metals and azetidine carboxylic acid in mammalian and avian cells (2). In heat shocked HeLa cells, HSP40 translocates rapidly from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (1). This suggests that HSP40 colocalizes with HSP70 and together they repair denatured proteins in the nuclei and nucleoli of heat shocked cells (1). This is analogous to the functions of bacterial DnaJ and DnaK which have been shown to act together in dissociating the bacteriophage lambda protein complexes (5,6) and monomerizing the Rep A dimer (7).
Applications
Suitable for use in Immunohistochemistry (paraffin), Immunofluorescence, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:5000 (Colorimetric), detects a band of ~40kD Immunoprecipitation: 1:50 Optimal dilutions to be determined by researcher.
Positive Controls
Recombinant Human Hsp40 Protein HeLa Heat Shocked Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human HSP40
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human HSP40. It does not detect DnaJ in E. coli lysates. Species Crossreactivity: Human, monkey, mouse, rat, hamster, guinea pig, rabbit, canine, bovine, sheep, porcine, chicken and fish.
