Technical Data

H1840-75
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG1
Clone Number
5i56
Grade
Affinity Purified
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Mouse Anti-Helicobacter pylori Urease, Small Subunit Antigen

H. pylori produces large amounts of the enzyme urease, molecules of which are localized inside and outside of the bacterium. Urease breaks down urea (which is normally secreted into the stomach) to carbon dioxide and ammonia. The ammonia is converted to ammonium by accepting a proton (H+), which neutralizes gastric acid. The survival of H. pylori in the acidic stomach is dependent on urease. The ammonia produced is toxic to the epithelial cells, and along with the other products of H. pylori—including proteases, vacuolating cytotoxin A (VacA), and certain phospholipases, damages those cells.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:100-1:2500 Western Blot: 1:100-1:2500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Highly purified H. pylori Urease, small subunit.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes Helicobacter pylori Urease, small subunit.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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