H1847-53X-ML490
Clone Type
MonoclonalHost
MouseConjugate
MaxLight™490Isotype
IgG2aClone Number
3G165Grade
Affinity PurifiedApplications
FLISA IC IF WBShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (MaxLight 490)
MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
The HA tag sequence is derived from influenza hemagglutinin and is typically used to facilitate the detection of proteins. Tags such as HA used in conjunction with proteins can aid in purification, identification, and functional analysis of the tagged protein.Through the use of standard molecular biology techniques, a cDNA, which encodes the protein of interest can be cloned, in- frame, into an expression vector containing the tag sequence. The resultant tagged protein is usually referred to as an epitope-tagged protein or a fusion protein. Currently, a variety of expression vectors containing tags are commercially available. Depending on the expression vector selected, these fusion proteins may be expressed in a variety of organisms including bacteria, yeast, insect cells, and mammalian cells. Detection of a tagged protein is facilitated by the use of an antibody directed against the particular tag. This alleviates the need to generate a specific antibody against the protein itself. Therefore, newly identified proteins can be expressed and studied without having to wait for a specific antibody to be generated. Some of the more commonly used fusion tags include: c-myc, FLAG, GFP, GST, HA, His, and MBP.
Applications
Suitable for use in FLISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive control
HA-tagged JNK-1 transfected cell lysates
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide containing the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes HA tagged fusion proteins. |Species Crossreactivity: broad
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Tu, Y., et al; Mol. Biol. Cell 9:3367-3382 (1998). 2. Terpe, K., Appl. Microbiol. Biotechnol. 60: 523-533 (2003). 3. Tu, Y., et al., Mol. Biol. Cell 9: 3367-3382 (1998). 4. Bhattacharya and Cabral, Mol. Biol. Cell 15(7): 3123-3131 (2004). 5. Yamanaka, et al., Curr. Biol. 13(9): 734-743 (2003). 6. Chang, et al., Genes Dev. 18(16): 2010-2023 (2004). 7. Munro, S., Pelham, H.R., EMBO J. 3: 3087-3093 (1984). 8. Madeo, F., et al., Mol. Cell 9: 911-917 (2002). 9. Ruckdeschel, K., et al., J. Immunol. 168: 4601-4611 (2002). 10. Field, J., et al., Mol. Cell. Biol. 8: 2159-2165 (1988). 11. Schirmer, E., et al., J. Cell Biol. 153: 479-490 (2001). 12. Bjerling, P., et al., Mol. Cell. Biol. 8: 2170-2181 (2002). 13. Wilson, l.A., et al., Cell 37: 767-78 (1984). 14. Muster, T., et al., J. Virol. 69: 6678-6686 (1995).USBio References
No references available