Technical Data

H1847-69N
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
D60G11
Grade
Purified
Applications
IP WB
Crossreactivity
Hu
Gene ID
3162
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HMOX1 (Heme Oxygenase 1, HO-1, HO, HO1)

Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Heme oxygenase comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Ala110 of human HO-1.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total human HO-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Abraham, N.G. and Kappas, A. (2008) Pharmacol Rev 60, 79-127. 2.Otterbein, L.E. et al. (2003) Trends Immunol 24, 449-55. 3.Cruse, I. and Maines, M.D. (1988) J Biol Chem 263, 3348-53. 4.Maines, M.D. (1988) FASEB J 2, 2557-68. 5.Schipper, H.M. et al. (2009) Curr Alzheimer Res 6, 424-30.
USBio References
No references available
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