Technical Data

H1890-90B
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Isotype
IgG1,k
Clone Number
6F166 (A74)
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Bo Hu
Gene ID
444872, 3339
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Heparan Sulfate Proteoglycan (Perlecan, HSPG)

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450kD and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (2).

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Can also be used in affinity chromatography, in conjunction with H1890-90A to separate recombinant domain I from full length perlecan. Other applications not tested.
Recommended Dilutions
ELISA: 1:2000 Western Blot: 1:100 Immunohistochemistry: 1:100. Frozen PLP-fixed sections of bovine and human tissues Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Bovine corneal endothelial cells. Epitope: domain V.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A/G affinity chromatography from culture supernatant.
Specificity
Highly specific for bovine perlecan. There is no evidence for crossreactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Whitelock, J.M., Murdoch, A.D., Iozzo, R.V., Underwood, P.A. (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086. 2. Whitelock, J.M., Graham, L.D., Melrose, J., Murdoch, A.D., Iozzo, R., Underwood, P.A. (1999) Human perlecan immunopurified from different endothelial cell sources has different adhesive properties for vascular cells. Matrix Biol 18:163-178. 3. Olsen, B.R. (1999) Life without perlecan has its problems. J Cell Biol 147:909-912.
USBio References
No references available
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