Rat Anti-Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Heparan sulfate proteoglycans (HSPGs) are found on cell surfaces and in the extracelluar matrix of all mammalian tissues, particularly in the core of the basement membrane. HSPGs found in the brain are dystroglycan, N-syndecan, glypican, and perlecan. The composition of HSPGs differs in the protein core, and is thought to determine the location of HSPG in the cell membrane (syndecan and glypican) and extracellular matrix (perlecan and dystroglycan). The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration and morphogenesis.
Heparan Sulfate Proteoglycan antibodies, in tissues, react strongly and uniformly with basement membranes. The reactivity is independent of the galactosaminoglycan moieties; therefore, the epitope is not sensitive to heparinase treatment.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1:100; Detected HSPG in large cell carcinoma tissue. Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher.
Positive Control
HeLa and A431 cells
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Heparan Sulfate Proteoglycan from EHS mouse tumor. Epitope: HSPG core protein.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Heparan Sulfate Proteoglycan (Perlecan). Recognizes domain IV of the core protein of the large Heparan Sulfate Proteoglycan. Does not crossreact with laminin, collagen IV, entactin/nidogen or fibronectin.
