Sheep Anti-Hepsin (TMPRSS1)
Hepsin (Transmembrane protease serine 1) is a 51kD member of the peptidase S1 family of enzymes. It is expressed by both hepatocytes and numerous tumor cells, and activates HGF plus pro-uPA. Human Hepsin is a type II transmembrane protein that is 417 amino acids (aa) in length. It contains a 23 aa N-terminal cytoplasmic region and a 373 aa extracellular domain (ECD) (aa 45-417). The ECD possesses a SRCR segment (aa 54-151) and a peptidase S1 domain (aa 163-405). Over aa 46-417, human Hepsin shares 90% and 96% aa identity with mouse and canine Hepsin, respectively.
Applications
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5 -1.0ug/ml detection limit for rhHepsin is ~ 3ng/well. Western Blot: 0.1-0.2ug/ml detection limit for rhHepsin is ~ 5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived rhHepsin extracellular domain. (aa 46 - 417)
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Hepsin.
