Rabbit Anti-HERG1, CT (Erg1, Kcnh2, Ether-a-go-go-related Channel 1, Voltage-gated Potassium Channel)
This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 5ug/ml Immunohistochemistry: 4% paraformaldehyde fixed tissues. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa1145-1159 from the C-terminus of human HERG1.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ERG1 at 175 and 200kD (glycosylated) also 135 and 165kD (deglycosylated). May also recognize a strong band at ~37kD. Species Crossreactivity: rat and mouse
