Technical Data

H2034-52C
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
5H14 (42s2)
Grade
Affinity Purified
Applications
ChIP IHC WB
Crossreactivity
Hu Mo
Accession #
NP_009207
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Heterochromatin Protein 1, gamma (HP1g, M32)

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1alpha, HP1beta and HP1gamma). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Applications
Suitable for use in Western Blot, Immunohistochemistry and Chromatin Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 0.01-0.1ug/ml detects HP1g protein in acid-extracted HeLa cell lysates. Immunohistochemistry: Reported to detect HP1g positive cells in breast tissue. Chromatin Immunoprecipitation (ChIP): Reported to immunoprecipitate HP1g from nuclear extracts and formalin-cross-linked chromatin. Optimal dilutions to be determined by the researcher.
Positive Control
HeLa acid extracts, HEK 293 nuclear extracts.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein corresponding to aa17-173 of human Heterochromatin Protein 1 gamma with GST.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human HP1g protein, MW ~25kD. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Zhao, T., et al. (2001). J. Biol. Chem. 276: 9512-9518. 2. Eissenberg, J.C., et al. (2000). Curr. Opin. Genet. Dev. 10: 204-210.
USBio References
No references available
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