Rabbit Anti-Hexosaminidase A. NT (Hexosaminidase Subunit A, HEXA, Beta-hexosaminidase Subunit alpha, Beta-N-acetylhexosaminidase Subunit alpha, MGC99608, N-acetyl-beta-glucosaminidase Subunit alpha, TSD)
Hexosaminidase A (alpha Polypeptide), EC=3.2.1.52
HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 142-170 in the N-terminal region of human Hexosaminidase A. Swiss/UniProt Accession: P06865.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human Hexosaminidase A. Species Crossreactivity: mouse
