Technical Data

H5090-01H
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)
Rabbit Anti-Histamine Receptor 1 (H1R) (Not for Export EU)

Histamine, one of the most important mediators of allergy and inflammation. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. Histamine effects are mediated by four pharmacologically distinct receptors, the H1R, H2R , H3R and H4R receptors. Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length. H1R (mouse 488 aa; rat 486 aa; human 487 aa, chromosome 3p21-p14; ~75-85% interspecies homology) is distributed in the brain, most smooth muscle cells, endothelial cells, adrenal medulla, and heart. H1R plays roles in smooth muscle contraction, stimulation of nitric oxide formation, endothelial cell contraction, and increasing vascular permeability, all of which have close relationships with allergic conditions. H1R preferentially couples to the Gq/11 family of G-proteins and causes mobilization of intracellular Ca 2+ in a pertussis toxin-insensitive fashion. Compounds such as mepyramine (pyrilamine) and triprolidine are highly potent H1 antagonists, and easily penetrate the brain causing sedation. Many new non-sedating H1 antagonists (e.g. cetirizine, etc), are used to treat allergic conditions).

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000. ELISA: 1:10,000-1:100,000 with the use of 50-100ng of control peptide/well (Cat #H5090-01F). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 19 aa peptide within the cytoplasmic loop # 3 (between TM 5 and 6) of human H1R. (Conjugated to KLH)
Form
Supplied as a liquid, 40% glycerol.
Purity
Serum
Specificity
Recognizes human Histamine Receptor 1 (H1R). The immunogen shows no significant sequence homology with other histamine receptors. Species sequence homology: fish and monkey-100%, rat and mouse-63%, guinea pig and bovine-57%.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Fujimoto K et al (1993) BBRC, 190, 294; Inoue I et al (1996) PNAS 93, 13316; DeBacker MD et al (1993) BBRC 197, 1601; Fukui H et al (1994) BBRC 201, 894; Moguilevsky N et al (1994) Eur. J. Biochem. 224, 489
USBio References
No references available
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