Technical Data

H5099-05A-ML650
Clone Type
Monoclonal
Host
Mouse
Conjugate
MaxLight™650
Isotype
IgG1,k
Clone Number
4A12E4
Grade
Affinity Purified
Applications
FLISA IP WB
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-His-Tag (6-Histidine) (MaxLight 650)

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

The His-tagged expression system is widely used in the production of recombinant protein. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Applications
Suitable for use in Immunoprecipitation, FLISA and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunoprecipitation: 10ug/application FLISA: 0.1-1ug/ml Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide containing six His residues (6xHis)
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Specific for 6xHis. On Western blots, it identifies a band at ~ 37kDa. Reactivity has been confirmed with TS-His fusion protein, and is expected to react with all His-tagged proteins regardless of species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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