Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Western Blot: 1:500-1:1000 Optimal working dilutions to be determined by researcher.

I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-65C: IgG, H&L (HRP) Pab Rb x Mo I1904-65H: IgG, H&L, X-Adsorbed (HRP) Pab Rb x Mo I1904-65N: IgG (HRP) Pab Rb x Mo I1903-07E: IgG (HRP) Pab Rb x Mo I1904-67R: IgG H&L, X-adsorbed (HRP) Pab Rb x Mo

NS1 myeloma cells with spleen cells from Balb/c mice.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

PAX6 transcription factor linked to 6-histidine tag (HHHHHH).

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. Has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian cell lines. In Western Blot of bacterial extracts, does not crossreact with any endogenous products, though some crossreactivity may be seen with extracts of insect or mammalian cells.