The His-tagged expression system is widely used in the production of recombinant proteins. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

Suitable for use in FLISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Optimal dilutions to be determined by the researcher.

NS1 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

PAX6 transcription factor linked to 6-histidine tag (HHHHHH).

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. Has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian cell lines. In Western Blot of bacterial extracts, does not crossreact with any endogenous products, though some crossreactivity may be seen with extracts of insect or mammalian cells.