Technical Data

H5099-16-PE
Clone Type
Monoclonal
Host
Mouse
Conjugate
PE
Isotype
IgG1
Clone Number
3H2201 (AD1.1.10)
Grade
Affinity Purified
Applications
FLISA IP WB
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-His-Tag (6-Histidine, 6 His, 6-His Epitope Tag, HHHHHH Epitope Tag, HHHHHH Tag, Polyhistidine Tag) (PE)
Histidine Tag

The His-tagged expression system is widely used in the production of recombinant proteins. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

Applications
Suitable for use in FLISA, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
PAX6 transcription factor linked to 6-histidine tag (HHHHHH).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. Has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian cell lines. In Western Blot of bacterial extracts, does not crossreact with any endogenous products, though some crossreactivity may be seen with extracts of insect or mammalian cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Conrath, K., et al., J. Biol. Chem. 276: 7346-7350 (2001). 2. Suen, J.L., et al., Immunol. 103: 301-309 (2001). 3. Hoffmann, S.C., et al., J. Biol. Chem. 282: 22,370-22,375 (2007). 4. Zheng, J., et al., BMC Immunol. 8: 4 (2007). 5. Bahi, A. & Dreyer, J.L., Genes Brain Behav. 7: 244-256 (2008). 6. Wrighton, K.H., et al., J. Biol. Chem. 284: 9755-9763 (2009). 7. Diefenbacher, M., et al., J. Biol. Chem. 286: 25,027-25,038 (2011). 8. Alvarez, M.M., et al., PLoS One 5: e10176 (2010). 9. Bahi, A., et al., Neuropsychopharmacology 33: 2726-2734 (2008). 10. Gunnarsen, K.S., et al., BMC Biotechnol. 10: 8 (2010). 11. Hwang, H.Y., et al., Mol. Immunol. 45: 2570-2580 (2008). 12. De Vooght, L. et al. (2012) Microb Cell Fact. 11: 23. 13. Saerens, D. et al. (2004) J Biol Chem. 279 (50): 51965-72. 14. Than, N.G. et al. (2014) Placenta. 35: 855-65.
USBio References
No references available
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