Mouse Anti-Histone H1, phosphorylated
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 detected Histone H1 in colcemid-treated HeLa lysate. Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate, treated with colcemid
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated bovine nuclear extract.
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity
Recognizes bovine phosphorylated Histone H1 at ~32kD. Species Crossreactivity: human and mouse, Broad species cross-reactivity is expected, based on sequence homology.
