H5110-12A3
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_003528Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Histone H3, phosphorylated (Ser28) (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser28 of human H3 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Histone H3 when phosphorylated at Ser28.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003). 2. Deng, L., et al., Virology 289(2):312-326 (2001). 3. Deng, L., et al., Virology 277(2):278-295 (2000). 4. El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998). 5. Albig, W., et al., Hum. Genet. 101(3):284-294 (1997).USBio References
No references available