H5110-14B-ML650
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
MaxLight™650Isotype
IgGGrade
Affinity PurifiedApplications
IC IP WBCrossreactivity
Dr Hu Th XeAccession #
NP_003484.1Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Rabbit Anti-Histone H3, phosphorylated (Ser10) (Mitosis Marker) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.5-1ug/ml detected histone H3on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using a goat anti-rabbit secondary antibody (HRP) and a chemiluminescence detection system. Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate. Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-47H: IgG, H&L (HRP) Pab GtxRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab GtxRt I1904-47T: IgG, H&L (HRP) Pab GtxRt I1904-50T1: IgG, H&L (HRP) Pab GtxRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab GtxRt I1904-40D: IgG, H&L (FITC) Pab Gt xRb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Linear peptide coresponding to human Histone H3 at Ser10
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Park, K.-S. et al., (2006) Am J Respir Cell Mol Biol. 34:151–157. 2. Akeno N., et al. 2014. Oncogene. 2014 Jan 27. doi: 10.1038/onc.2013.589. [Epub ahead of print].|Other references:|1. Adams, R.R., et al., J. Cell Biol. 153: 865-880 (2001). 2. Canela, N., et al., J. Biol. Chem. 278: 1158-1164 (2003). 3. Seigel, P.M. et al., Proc Natl. Acad. Sci. USA 100: 8430-8435 (2003). 4. Wei, Y., et al., Cell 97: 99-109 (1999) 5. Miller, L.D., et al., Developmental Dynamics 231: 57-71 (2004). 6. Chadee, D.N., et al., J. Biol. Chem. 270: 20,098-20,105 (1995). 7. Ajiro, K., et al., J. Biol. Chem. 271: 13,197-13,201 (1996). 8. Mahadevan, L.C., et al., Cell 65: 775-783 (1991). 9. Hendzel, M.J., et al., Chromasoma 106: 348-360 (1997).USBio References
US Biological application reference: Park, K.-S. et al., (2006) Am J Respir Cell Mol Biol. 34:151–157. 2. Akeno N., et al. 2014. p53 suppresses carcinoma progression by inhibiting mTOR pathway activation. Oncogene. 2014 Jan 27. doi: 10.1038/onc.2013.589. [Epub ahead of print].